Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection [1995](IR94).doc

Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection [1995](IR94).doc

Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection [1995](IR94)

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Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection [1995](IR94)

http://highwire.stanford.edu/cgi/medline/pmid;8749248?maxtoshow=&HITS=&hits=&RESULTFORMAT=&fulltext=Effect+of+ageing+on+human+plasma+glutathione+concentrations&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT
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Effect of ageing on human plasma glutathione concentrations as determined by high-performance liquid chromatography with fluorimetric detection.
CS Yang, ST Chou, L Liu, PJ Tsai, and JS Kuo
J Chromatogr B Biomed Appl, December 1, 1995; 674(1): 23-30.    

Department of Medical Research. Taichung Veterans General Hospital Taiwan (台中榮民總醫院).

A convenient method for the determination of reduced glutathione (GSH) and oxidized glutathione (GSSG) in human plasma by high-performance liquid chromatography with fluorescence detection is reported. This assay involves direct addition of human plasma to methanolic monobromobimane, for simultaneous protein precipitation and thiol derivatization. The assay was validated by addition of authentic GSH and GSSG to plasma samples. Plasma glutathione levels in Chinese male and female volunteers were found to decrease with increasing age (age groups 20-30, 30-40, 40-50, 50-60, and > 60; mean +/- S.E.M. 0.95 +/- 0.03, 0.77 +/- 0.02, 0.67 +/- 0.03, 0.51 +/- 0.02, 0.48 +/- 0.02 microM for male volunteers and 1.11 +/- 0.06, 0.76 +/- 0.03, 0.61 +/- 0.03, 0.53 +/- 0.04 and 0.43 +/- 0.04 microM for female volunteers). GSSG levels, in both males and females, did not show a correlation with age. There were no significant differences in GSH or GSSG levels among male and female volunteers of the same age group. These results suggest that elderly persons might be more susceptible to oxidative injury due to decreased plasma glutathione levels.

Publication Types:

Journal article
Research support, non-u.s. gov't
MeSH Terms:

Adult
Aged
Aging
Bicyclo Compounds
Chromatography, High Pressure Liquid*
Dithiothreitol
Ethylmaleimide
Female
Fluorometry
Glutathione
Humans
Male
Middle Aged
Reproducibility of Results
Sulfhydryl Reagents
PMID: 8749248

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